McGlynn doesn't believe men should run out and stock up on pregnancy tests. Choriocarcinomas are, after all, very rare. "Only about 2 men in 100,000 will get any kind of testicular cancer," explains KoerthBaker, "[and] pure choriocarcinomas the dangerous kind that I'm talking about here make up less than 1% of those diagnoses.".Immunofluorescence analysis confirmed that myctagged Notch1LNG was detected at the membrane as expected (Figure 2d, inset). Furthermore, by immunoblot analysis, both myctagged constructs were expressed at comparable levels with increased amounts of processed NICD (85 generated in Notch1LNG CCSStransfected cells (Figure 2d inset). Cells undergoing neglectinduced apoptosis showed a loss of adherence to substrate, which was apparent in the rounded morphology of cells undergoing neglect and disorganization of mitochondria (Figure 2e, GFP).

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